The design work to estimate and analyze the signal shape and quality at the receiving circuit is called Signal Integrity (SI) analysis. One possible method is to measure the signal shape and signal eye diagram to see that the signal levels and rise/fall times are fast enough. The measurement is not always possible because traces start & stop under the components and travel in inner layers. The simulation is the only way in these cases.
A disadvantage of this after-layout work approach is that the design is already done when you have the results. This is the reason we split the work into pre-layout and post-layout phases. In the pre-layout phase, the interesting details on the signal path are analyzed beforehand and design guides are generated for the layout designers. When the post-layout work is about analyzing the design that is already done.